Autocycler Express
The Problem: Generating perfect, complete bacterial genome assemblies from long-read sequencing data is challenging. Automated assemblers often make errors like duplicating plasmids or failing to circularize sequences. While manual curation tools exist, they are typically labor-intensive and require command-line expertise, creating a bottleneck as sequencing output grows.
Our Approach: Autocycler Express is a browser-based user interface that streamlines and simplifies the manual curation of genome assemblies using the Autocycler pipeline. It allows for a rapid, iterative process of visualizing assembly graphs, identifying contigs (e.g., via BLAST), removing bad clusters, and cleaning the graph. This approach empowers biologists without programming skills to participate in the curation process, dramatically reducing manual effort while achieving high-quality, closed genomes ready for submission.